This study aims to investigate prosocial behavior of students attending pre-school education institutions. Survey research was used in the study. The sample of the research included 325 children attending five different pre-school education institutions in the 2020-2021 academic year. The participants were determined with the convenience sampling type of purposive sampling method. The data on the prosocial behavior levels of the students attending pre-school education institutions were collected by using the “Child Prosociality Scale” Mother Form. The data were analyzed with package program. The research findings revealed that students prosocial behavior attending pre-school education institutions prosocial behavior levels were high and students prosocial behavior don’t significantly differ in terms of gender and age variables. According to the duration of attending a pre-school education institution variable, the prosocial behavior mean scores of students who attended a pre-school education institution for 3 years were found higher than scores of those who attended a pre-school education institution for 1 year. It was revealed that the mother’s age and education level did not differ significantly prosocial behavior of the children.