The aim of the present study was to understand how gender perceptions of Turkish prospective teachers differ in terms of socio-demographic factors. The study sample consisted of 1075 Turkish prospective teachers (738 females, 68.7%; 337 males, 31.3%) Stratified random sampling technique was used to recruit a representative sample. Perception of Gender Scale and Demographic Information Form were used as measuring instruments. Independent-samples t-test and one-way analysis of variance (ANOVA) were performed to test research hypotheses. The results of independent-samples t-test differed according to gender and working status of mother. However, gender perceptions of prospective teachers did not differed according to family structure. The results of ANOVA tests indicated that gender perceptions of prospective teachers did not differ in terms of age groups and perceptions regarding residential settlement. Nevertheless, it was determined that residential settlement where participants lived most, monthly family income, family type, parental educational levels had significant influences on gender perceptions of prospective teachers. The findings and limitations are discussed, suggestions for future research are provided.