This study attempts to investigate the sources of stress experienced by thepre-service teachers during musical instrument education. The study employed qualitative research design. The sample of the study consisted of 36 students studying at the 1st, 2nd, 3rd and 4th grades in the Music Teaching Program of Fine Arts Teaching Department of a state university in the 2017-2018 academic year. In the study, the structured interview technique was used to collect data. In attempts to collect the data, the pre-service teachers were asked to answer “What are the sources of stress you experience in musical instrument education?”.Content analysis was used for the analysis of qualitative data. Considering the pre‐service music teachers’ responses, the sources of stress perceived by the pre-service music teachers during musical instrument education were grouped into three categories: performance stress, affective stress and physiological stress. As a result of the study the sourcess of stress experienced by the pre-sevice music teachers in musical instrument education were discussed and a series of suggestion presented. It is thougt that the study will shed light on similiar researchers and contribute to development of further studies aiming at reducing the effects of the stressors the pre-sevice music students experience in musical instrument education.