This study aims to determine the universal science literacy levels of primary school pre-service teachers studying at Bursa Uludağ University and Sakarya University and to examine how these levels change according to some variables (such as gender, education level, and parental education levels…). For this purpose, the Universal Science Literacy Scale (USLS) was applied to 214 pre-service teachers. This research, in which the quantitative research design is used, is in the survey model. The SPSS package program was used in the analysis of the data obtained from the research. As a result of the analysis of the data, it was determined (Classification, 201.7-240 score range was determined as “very high”, X̅=203) that the primary school pre-service teachers’ universal science literacy level is "very high". In addition, it was determined that the gender variable affected the USL levels of teacher candidates and this effect was in favor of women. It was found that the level of education, the environment in which the education was completed, the type of high school graduated from, and the education level of the parents did not have a statistically significant effect on the USL levels of the classroom teacher candidates. The fact that universal science literacy level has been found to be very high might be indicating that science education in our country is given relating it with the daily life and using the laboratory environment frequently.